Simultaneous determination of the concentration of phenytoin and phenobarbital in normal human plasma by high-pressure liquid chromatography 应用高压液相色谱分析法同时微量测定人血浆中苯妥英钠和苯巴比妥的浓度
It is concluded that the recombinant plasmid RC/ RSV hF ⅷ BDcDNA can successfully express human F ⅷ in mouse 32D cell line, and hF ⅷ expressed in vitro presents the similar coagulant activity to the native hF ⅷ existing in normal human plasma. 结论:非病毒质粒载体pRC/RSV介导的人凝血因子Ⅷ基因能够在小鼠32D细胞系中表达人FⅧ蛋白,所表达的FⅧ与正常人血浆中的野生型FⅧ具有相似的凝血活性。
Methods: The antithrombin mechanism of Sjamp was checked by assaying its effects on the thrombin activity in normal human pooled plasma, purified human heparin cofactor ⅱ ( HC ⅱ) system and antithrombin ⅲ ( AT ⅲ) system. 方法:用国产Sjamp作为激动剂,在正常混合血浆体系、纯化的肝素辅因子Ⅱ(HCⅡ)体系以及纯化的抗凝血酶Ⅲ(AT-Ⅲ)体系研究Sjamp的抗凝血酶作用机制。
Objective To study the determination of biological activity of α_1-AT with chromogenic substrate and its influential factors using fresh pooled normal human plasma as reference standard. 目的研究用发色底物法测定α1-AT生物活性时,以正常人混合血浆为参考标准的血浆稀释度范围和测定的影响因素。